Pregnancy Testing – OSCE Guide

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Performing a pregnancy test using a pregnancy testing strip can occasionally show up as an OSCE station and you’ll be expected to confidently carry out the procedure. This pregnancy testing OSCE guide will provide a step-by-step approach to performing this clinical skill.

Pregnancy testing revolves around the detection of human chorionic gonadotropin levels (hCG) in the urine, which can normally be detected 7-10 days after fertilisation has occurred.


Gather equipment

Gather the equipment you’ll need to perform pregnancy testing:

  • Alcohol gel
  • Gloves
  • Apron
  • Pregnancy test strip
  • Labelled urine sample
  • Paper towels

Different clinical areas may use different pregnancy test strips, however, the principles remain the same.


Procedure

1. Wash your hands and don PPE

2. Confirm the patient’s details on the sample bottle are correct including their name, date of birth and hospital number

3. Ensure the urine sample collected is fresh and is at room temperature

4. Check the expiry date of the pregnancy test and ensure this is within date

5. Remove the test strip from the protective packet immediately before use

6. Label the test strip with the patient’s details

7. Submerge the test strip in the urine sample vertically until the ‘MAX’ line is reached

8. Submerge for a minimum of 10-15 seconds

9. Remove the strip from the urine sample and tap off excess urine using the side of the bottle

10. Place the test strip on a paper towel on a flat surface

11. Wait 3 minutes (or according to the package guidelines)

12. Interpret the test strip

13. Dispose of the test strip and PPE appropriately into a clinical waste bin

14. Wash your hands

Interpreting a pregnancy test

The control line must always be present to confirm a valid test.

A single red line in the control region indicates a negative test.

Two red lines indicate a positive test (even if the second line is faint).

No red lines indicate an invalid test, which requires repeating.

Pregnancy testing examplePregnancy testing example


To complete the procedure…

Summarise your findings.

Document your findings, including the date and time of the test, the test result, your name and grade, and your signature.

Suggest further investigations based on the result and clinical context.

Further investigations
  • Serum hCG: to quantify the level of hCG, helping to monitor the pregnancy’s progression
  • Pelvic ultrasound: if an ectopic pregnancy is suspected, to confirm whether the pregnancy is intrauterine or not 
  • Transvaginal ultrasound: to aid with the detection of a fetal heartbeat, determining gestational age, viability and chorionicity

Editor

Dr Jamie Scriven


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